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help/submission/physical
There are two possibilities to submit samples to CCG (Center for Chemical Genetics)
- Solution (preferential)
- All compounds should be preferentially submitted in solution, dissolved in DMSO or H2O and placed in 96 well plates and sealed.
- Concetration of the solution should be 10 mmol/L (i.e. 0.01 mol/L)
- Volume of the solution should be 250 - 1000 µL
- The last column of the plate (i.e. wells labeled A12-H12) must be left empty (i.e. there can be max 88 samples per plate)
- Recommended plates: Nunc 260252 (96 DeepWell Round Bottom Plate, Low Profile, Non Strile, PP 5/SL;
www.nuncbrand.com).
For barcoded samples use Thermo Scientific 3741 (Matrix 1.0 ml, 2D barcoded, V bottom, w/caps, ScrewTop tubes in Latch Rack case, 96 tubes/case. http://www.thermoscientific.com) - Recommended seal tape: Costar 6570 (Thermowell Sealing Tape For 96 Well Plates Aluminum 100/Pack, Non-Sterile; www.scienceproducts.corning.com)
- Recommended DMSO: Sigma-Aldrich 41640 (DMSO puriss. p.a., ACS reagent http://sigmaaldrich.com)
- Solid (for number of samples less than 50)
- The amount of the sample (placed in a glass vial) should be around 1 mg (weighted exactly with precission 0.1 mg), so that after dissolving in 250-1000 µl the concentration will be 10 mmol/L
Information about the samples (structures and/or molecular weight, concentration, plate position, ...) must be submitted in the recommended format.